Human precision-cut cystic duct and gallbladder slices: a novel method for studying cholangiopathies

Background and aims Precision-cut tissue slices (PCTS) are widely used as an ex vivo culture technique to study pathogeneses of diseases drug activities in organs vitro . A novel application the PCTS model may be field translational research into cholangiopathies such biliary atresia. Therefore, aim this was apply precision-cut slice human bile duct gallbladder tissue. Methods Cystic derived from patients undergoing a cholecystectomy were collected, preserved for preparation cystic (PCCDS) (PCGS). The PCCDS PCGS prepared using mechanical slicer subsequently incubated 24 48 h respectively William's Medium E (WME) medium. Viability assessed based on ATP/protein content morphology [hematoxylin eosin (H&E) staining]. Results It shown that viability, by morphology, ( n = 8) could maintained over incubation period respectively. increased significantly 0.58 ± 0.13 pmol/µg at 0 2.4 0.29 after P .0003). similar significant increase 0.94 0.22 3.7 0.41 .0005) 4.2 0.47 .0002) observed PCGS. Morphological assessment showed viable Conclusion This is first report use remain least h, which makes them suitable purposes cholangiopathies, including